期刊
DIABETES
卷 69, 期 12, 页码 2589-2602出版社
AMER DIABETES ASSOC
DOI: 10.2337/db20-0262
关键词
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资金
- Ontario Graduate Scholarship program
- Banting and Best Diabetes Centre (University of Toronto)
- Canadian Institutes of Health Research [PJT-15308]
- Ontario Graduate Scholarship
- Banting and Best Diabetes Centre
- Biological Rhythms Training Program (University of Toronto)
- Tier II Canada Research Chair
- John P. Mitchell Cancer Award from the Undergraduate Research Opportunity Program, University of Toronto
- Tier I Canada Research Chair
- Banting and Best Diabetes Centre (Pilot and Feasibility Grant)
- 3D (Diet, Digestive Tract and Disease) Centre - Canadian Foundation for Innovation
- Ontario Research Fund [19442, 30961]
The incretin glucagon-like peptide 1 (GLP-1) is secreted by the intestinal L cell upon nutrient ingestion. GLP-1 also exhibits a circadian rhythm, with highest release at the onset of the feeding period. Similarly, microbial composition and function exhibit circadian rhythmicity with fasting-feeding. The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice. In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected. Furthermore, the changes in GLP-1 secretion were shown to be essential for the maintenance of normoglycemia in this obesogenic environment. Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion. Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release. In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
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