期刊
DEVELOPMENTAL CELL
卷 54, 期 5, 页码 583-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2020.08.004
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资金
- Centre National de la Recherche Scientifique (CNRS)
- European Union (ERC) [340784]
- ARC [SL220130607097]
- Francis Crick Ph.D. Training Programme [FC001204]
- Fondation ARC pour la Recherche sur le Cancer [PDF20180507510]
- European Research Council (ERC) [340784] Funding Source: European Research Council (ERC)
Planar cell polarity (PCP) organizes the orientation of cellular protrusions and migratory activity within the tissue plane. PCP establishment involves the subcellular polarization of core PCP components. It has been suggested that Wnt gradients could provide a global cue that coordinates local PCP with tissue axes. Here, we dissect the role of Wnt ligands in the orientation of hairs of Drosophila wings, an established system for the study of PCP. We found that PCP was normal in quintuple mutant wings that rely solely on the membrane-tethered Wingless for Wnt signaling, suggesting that a Wnt gradient is not required. We then used a nanobody-based approach to trap Wntless in the endoplasmic reticulum, and hence prevent all Wnt secretion, specifically during the period of PCP establishment. PCP was still established. We conclude that, even though Wnt ligands could contribute to PCP, they are not essential, and another global cue must exist for tissue-wide polarization.
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