期刊
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
卷 22, 期 8, 页码 1122-1128出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1389201021999201001204345
关键词
Wounds; Aloe vera; skin; angiogenesis; hydroxyproline; herbs
资金
- HEC under NRPU project [7517/Punjab/NRPU/RD/HEC/2017]
The study found that oral administration of Aloe vera promotes early healing of excisional wounds in Sprague Dawley rats, with gradual improvement in wound areas and collagen content. Aloe vera also increased the expression of IGF-1 and VEGF in skin and bone marrow, indicating enhanced angiogenesis. RT-PCR analysis showed upregulation of genes related to chemotaxis in skin and bone marrow.
Background: Aloe vera has been reported as a topical antibiotic and healing agent for wounds, but advantages of oral administration and mechanisms of wound healing have not been reported. Present study focuses on the evaluation of effects of oral administration of Aloe vera for excisional cutaneous wounds in Sprague Dawley rats. Methods: Sprague Dawley (SD) rats were inflicted with excisional wounds and were either treated with Aloe vera orally (Aloe vera) or kept untreated (wound). In contrast, healthy rats were kept as control group. Wound area was measured from day 7th to day 21st. Collagen content was estimated by hydroxyproline assay. Histology was analysed by hematoxylin and eosin staining. Angiogenesis was observed by indirect ELISA for Insulin like Growth Factor (IGF-1) and Vascular Endothelial Growth Factor (VEGF) protein from skin, serum and bone marrow. Chemotaxis was evaluated by RT-PCR analysis for Stromal cell-derived factor-1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR-4) from skin and bone marrow. Results: Aloe vera healed wounds earlier than untreated rats with gradual improvement in wound areas and collagen content. Aloe vera also improved the expression of IGF-1 and VEGF in skin and bone marrow indicating an improvement in angiogenesis. RT-PCR analysis showed increased expression of genes for chemotaxis (SDF-1 and CXCR-4) in skin and bone marrow. Conclusion: Aloe vera improves healing by increasing collagen content, improving angiogenesis and chemotaxis.
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