期刊
CURRENT GENE THERAPY
卷 21, 期 1, 页码 81-88出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1566523220666201005111126
关键词
Mesenchymal stem cells; caspase 8 and 9; apoptosis; GSK-3 alpha/beta and ERK1/2 signaling pathways; stem cell-based therapy
资金
- University of Tabriz [S/1712]
The study found that MSCs co-cultured with Molt-4 cells could promote Molt-4 cell apoptosis and cell senescence through certain signaling pathways.
Background: Mesenchymal stern cells (MSCs) are considered an interesting tool in cell therapy due to their unique features such as self-renewal, multi-potency, and pluripotency. The multifunctional properties of these cells are being investigated in many studies. The current research examined the influence of MSCs on the Molt-4 cell line as acute lymphoblastic leukemia (ALL) cells. Methods: MSCs were cultured, characterized, and co-cultured with Molt-4 cells in a trans-well system. Then, cultured Molt-4 alone and Molt-4 co-cultured with MSCs (10:1) were collected on day 7 and subjected to western blotting for protein expression assessment. Telomerase activity as well as cell senescence, were investigated by PCR-ELISA TRAP assay and beta-galactosidase activity measurement, respectively. Results: It was found that MSCs resulted in a significant increase in the pro-caspase-8 and cleaved-caspase 8 and 9 expression levels. Furthermore, protein expression levels of GSK-3 alpha/beta and ERK1/2 were significantly decreased. The results also showed that MSCs caused significant decreases and increases in telomerase and beta-galactosidase enzyme activity of Molt-4 cells, respectively. Conclusion: It was concluded that MSCs co-cultured with Molt-4 cells could be involved in the promotion of Molt-4 cell apoptosis and cell senescence via caspase-8, 9 cascade and OSK-3 alpha/beta and ERK1/2 signaling pathways.
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