Molecular advancements on over-expression, stability and catalytic aspects of endo-β-mannanases

The hydrolysis of mannans by endo-beta-mannanases continues to gather significance as exemplified by its commercial applications in food, feed, and a rekindled interest in biorefineries. The present review provides a comprehensive account of fundamental research and fascinating insights in the field of endo-beta-mannanase engineering in order to improve over-expression and to decipher molecular determinants governing activity-stability during harsh conditions, substrate recognition, polysaccharide specificity, endo/exo mode of action and multi-functional activities in the modular polypeptide. In-depth analysis of the available literature has also been made on rational and directed evolution approaches, which have translated native endo-beta-mannanases into superior biocatalysts for satisfying industrial requirements.

Automated summary

This review provides a comprehensive overview of research on endo-beta-mannanase engineering, covering key factors such as activity stability, substrate recognition, and mode of action, as well as rational and directed evolution approaches to improve industrial biocatalysts.