期刊
CLINICAL SCIENCE
卷 134, 期 19, 页码 2549-2563出版社
PORTLAND PRESS LTD
DOI: 10.1042/CS20200598
关键词
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资金
- National Natural Science Foundation of China [81903886, 81730110, 81973544, 81573671]
- Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme [GDHVPS2018]
- Guangdong Basic and Applied Basic Research Foundation [2019A1515010821]
- Young Elite Scientists Sponsorship Program by CACM [2019-QNRC2-C14]
- Guangzhou Education Bureau University Scientific Research Project [201831845]
Acute lung injury (ALI) is a life-threatening disease without effective pharmacotherapies, so far. Forsythia suspensa is frequently used in the treatment of lung infection in traditional Chinese medicine. In search for natural anti-inflammatory components, the activity and the underlying mechanism of Forsythoside A (FA) from Forsythia suspensa were explored. In the present paper, BALB/c mice and murine RAW 264.7 cells were stimulated by LPS to establish inflammation models. Data showed that FA inhibited the production of TNF-alpha and IL-6 and the activation of STAT3 in LPS-stimulated RAW 264.7 cells. Additionally, FA increased the expression level of microRNA-124 (miR-124). Furthermore, the inhibitory effect of FA on STAT3 was counteracted by the treatment of miR-124 inhibitor. Critically, FA ameliorated LPS-induced ALI pathological damage, the increase in lung water content and inflammatory cytokine, cells infiltration and activation of the STAT3 signaling pathway in BALB/c mice. Meanwhile, FA up-regulated the expression of miR-124 in lungs, while administration with miR-124 inhibitor attenuated the protective effects of FA. Our results indicated that FA alleviates LPS-induced inflammation through up-regulating miR-124 in vitro and in vivo. These findings indicate the potential of FA and miR-124 in the treatment of ALI.
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