期刊
CLINICAL AND EXPERIMENTAL ALLERGY
卷 50, 期 12, 页码 1325-1341出版社
WILEY
DOI: 10.1111/cea.13722
关键词
fungal sensitisation; fungal colonisation; high-throughput sequencing; mycobiome
资金
- NIHR Leicester Biomedical Research Centre
- Midlands Asthma and Allergy Research Association (MAARA)
- Asthma UK [AUK-PHD-2013-250]
- Henry Smith Charity
- Academy of Medical Sciences [SBF003\1125]
- Wellcome Trust
- Government Department of Business, Energy and Industrial Strategy
- British Heart Foundation
Background Fungal involvement in asthma is associated with severe disease. The full spectrum of fungal species in asthma is not well described and is derived largely from insensitive culture techniques. Objectives To use high-throughput sequencing to describe the airway mycobiota in asthmatics with and without fungal sensitization and healthy controls; to compare samples representing different airway compartments; to determine whether the mycobiota was influenced by the fungal composition of outdoor air; and to compare findings with clinically relevant outcomes. Methods We amplified the internal transcribed spacer region 2 of the nuclear ribosomal operon to identify the fungal species present. Ninety-seven subjects were recruited and provided sputum (83 asthmatics; 14 healthy subjects), with 29 also undergoing a bronchoscopy. A subset of airway samples were compared with matched outdoor air and mouthwash samples. Results Two hundred and six taxa at the species level were identified in sputum, most at low relative abundance.Aspergillus fumigatus,Candida albicansandMycosphaerella tassianahad the highest relative abundances and were the most prevalent species across all subjects. The airway mycobiota consisted of a complex community with high diversity between individuals. Notable shifts in the balance of fungi detected in the lung were associated with asthma status, asthma duration and biomarkers of inflammation.Aspergillus tubingensis, a member of theAspergillus nigerspecies complex, was most prevalent from bronchoscopic protected brush samples and significantly associated with a low sputum neutrophilia.Cryptococcus pseudolongus, from theCryptococcus humicolaspecies complex, was more abundant from bronchoscopy samples than sputum, and differentially more abundant in asthma than health. Conclusions and Clinical Relevance The airway mycobiota was dominated by a relatively small number of species, but was distinct from the oropharyngeal mycobiota and air samples. Members of theA. nigerandC. humicolaspecies complexes may play unexpected roles in the pathogenesis of asthma.
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