期刊
CHIRALITY
卷 32, 期 11, 页码 1289-1298出版社
WILEY
DOI: 10.1002/chir.23273
关键词
chiral mechanisms; docking and CD analysis; mandelic acid enantiomers; vancomycin stationary phase
资金
- Center for International Scientific Studies and Collaboration (CISSC) [1462]
So far, no detailed view has been expressed regarding the interactions between vancomycin and racemic compounds including mandelic acid. In the current study, a chiral stationary phase was prepared by using 3-aminopropyltriethoxysilane and succinic anhydride to graft carboxylated silica microspheres and subsequently by activating the carboxylic acid group for vancomycin immobilization. Characterization by elemental analysis, Fourier transform infrared spectroscopy, solid-state nuclear magnetic resonance, and thermogravimetric analysis demonstrated effective functionalization of the silica surface.RandSenantiomers of mandelic acid were separated by the synthetic vancomycin column. Finally, the interaction between vancomycin andR/Smandelic acid enantiomers was simulated by Auto-dock Vina. The binding energies of interactions betweenRandSenantiomers and vancomycin chiral stationary phase were different. In the most probable interaction, the difference in mandelic acid binding energy was approximately 0.2 kcal/mol. In addition, circular dichroism spectra of vancomycin interacting withRandSenantiomers showed different patterns. Therefore,RandSmandelic acid enantiomers may occupy various binding pockets and interact with different vancomycin functions. These observations emphasized the different retention ofRandSmandelic acid enantiomers in vancomycin chiral column.
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