Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis

The use of a microscale atmospheric pressure plasma jet (mu APPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the mu APPJ offered significantly higher H(2)O(2)production rates and better handling of larger reaction volumes. The performance of the mu APPJ was evaluated with recombinant unspecific peroxygenase fromAgrocybe aegerita(rAaeUPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the mu APPJ. The enzyme had a turnover of 36,415 mol mol(-1)and retained almost full activity even after prolonged plasma treatment. Overall, the mu APPJ presents a promising plasma source for plasma-driven biocatalysis.