期刊
CELL DEATH AND DIFFERENTIATION
卷 28, 期 2, 页码 715-729出版社
SPRINGERNATURE
DOI: 10.1038/s41418-020-00618-6
关键词
-
资金
- National Natural Science Foundation of China [81672589, 81971341, 81902621]
- China Postdoctoral Science Foundation [2019M662868, 2019M662867]
The high levels of miR-142-5p in advanced CSCC positively correlate with IDO expression in tumor-associated lymphatic vessels. The miR-142-5p is transferred by CSCC-secreted exosomes into LECs to induce IDO expression via ARID2-DNMT1-IFN-gamma signaling, leading to CD8(+)T cell exhaustion. This study suggests that LECs play a crucial role as immune checkpoints in the TME and may be a potential new target for CSCC diagnosis and treatment.
Clinical response to immunotherapy is closely associated with the immunosuppressive tumour microenvironment (TME), and influenced by the dynamic interaction between tumour cells and lymphatic endothelial cells (LECs). Here, we show that high levels of miR-142-5p positively correlate with indoleamine 2,3-dioxygenase (IDO) expression in tumour-associated lymphatic vessels in advanced cervical squamous cell carcinoma (CSCC). The miR-142-5p is transferred by CSCC-secreted exosomes into LECs to exhaust CD8(+)T cells via the up-regulation of lymphatic IDO expression, which was abrogated by an IDO inhibitor. Mechanistically, miR-142-5p directly down-regulates lymphatic AT-rich interactive domain-containing protein 2 (ARID2) expression, inhibits DNA methyltransferase 1 (DNMT1) recruitment to interferon (IFN)-gamma promoter, and enhances IFN-gamma transcription by suppressing promoter methylation, thereby leading to elevated IDO activity. Furthermore, increased serum exosomal miR-142-5p levels and the consequent IDO activity positively correlate with CSCC progression. In conclusion, exosomes secreted by CSCC cells deliver miR-142-5p to LECs and induce IDO expression via ARID2-DNMT1-IFN-gamma signalling to suppress and exhaust CD8(+)T cells. Our study suggests that LECs act as an integral component of the immune checkpoint(s) in the TME and may serve as a potential new target for CSCC diagnosis and treatment.
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