期刊
CALCIFIED TISSUE INTERNATIONAL
卷 108, 期 3, 页码 364-376出版社
SPRINGER
DOI: 10.1007/s00223-020-00772-6
关键词
MicroRNA; Extracellular vesicles; Osteoclasts; Myoblasts; Muscle; bone interaction
资金
- Japan Osteoporosis Foundation
- Osaka Medical Research Foundation for Intractable Diseases
- Cooperative Research Program (Joint usage/Research Center program) of Institute for Frontier Life and Medical Sciences, Kyoto University
- JSPS KAKENHI [19K18480]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [15H05935, C:20K09514]
- 2019 Kindai University Research Enchancement Grant [SR03]
- Grants-in-Aid for Scientific Research [19K18480, 20K09514] Funding Source: KAKEN
The interaction between muscle and bone was found recently, with extracellular vesicles playing a crucial role by transferring miRNA. The miR-196a-5p was identified to suppress osteoclast-like cell formation in mouse cells and affect mitochondrial energy metabolism.
Muscle/bone interaction has been recently noted. Extracellular vesicles (EVs) play a vital role in physiological and pathophysiological processes by transferring microRNA (miRNA) to distant tissues. We previously reported that EVs secreted from C2C12 myoblasts (Myo-EVs) suppress osteoclast differentiation. In the present study, we identified 4 miRNAs in Myo-EVs that suppressed osteoclast-like cell formation in Raw264.7 cells using small RNA sequencing analysis. Among them, miR-196a-5p expression was higher in C2C12 cells compared to mouse osteoblasts and bone marrow cells. Transfection of miR-196a-5p mimic suppressed the mRNA levels of osteoclast-related genes and mitochondrial energy metabolism induced by receptor activator of nuclear factor-kappa B ligand in Raw264.7 cells. In contrast, miR-196a-5p mimic enhanced osteoblastic differentiation in ST-2 cells and MC3T3-E1 cells. In conclusion, we demonstrated that miR-196-5p suppresses osteoclast-like cell formation and mitochondrial energy metabolism in mouse cells, suggesting that it might be a crucial factor for muscle/bone interaction via EVs.
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