期刊
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
卷 105, 期 4, 页码 620-625出版社
SPRINGER
DOI: 10.1007/s00128-020-02977-9
关键词
Ergosterol; Azole fungicides; qPCR; Freshwater fungi; Ascomycetes; Community composition
资金
- German Research Foundation, Project AQUA-REG (DFG) [SCHU227/14-1]
Due to their ecological importance, fungi are suitable indicator organisms for anthropogenic stress. To estimate fungal biomass, the fungal membrane molecule ergosterol is often quantified as a proxy. Estimates based on ergosterol may, however, be distorted by exposure to demethylase inhibiting (DMI) fungicides, interfering with sterol synthesis. To test this hypothesis, we exposed ten fungal species to the DMI fungicide tebuconazole and measured concentrations of ergosterol and DNA per unit dry mass of the fungal hyphae. The latter served as alternative biomass proxy that is not specifically targeted by tebuconazole. Effects of tebuconazole on ergosterol concentrations were species-specific, while concentrations were on average reduced by 13%. In contrast, DNA concentrations were on average increased by 13%. We demonstrate that DMI fungicides - at close to field relevant levels - can distort fungal biomass estimation, complicating the use of this endpoint for environmental management.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据