期刊
BRITISH JOURNAL OF NUTRITION
卷 125, 期 1, 页码 50-61出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114520002639
关键词
Choline; Inflammatory bowel disease; Colitis; Citrobacter rodentium
资金
- Natural Sciences and Engineering Research Council of Canada [436154, 03932]
- Alberta Innovates-Technology Futures
- Canada Research Chair Program
The study demonstrates the importance of dietary choline for patients with inflammatory bowel disease, as inadequate intake can worsen the severity of colitis and impact the structure of the gut microbiota.
Dietary choline, which is converted to phosphatidylcholine (PC) in intestinal enterocytes, may benefit inflammatory bowel disease patients who typically have reduced intestinal choline and PC. The present study investigated the effect of dietary choline supplementation on colitis severity and intestinal mucosal homoeostasis using a Citrobacter rodentium-induced colitis model. C57BL/6J mice were fed three isoenergetic diets differing in choline level: choline-deficient (CD), choline-sufficient (CS) and choline-excess (CE) for 3 weeks prior to infection with C. rodentium. The effect of dietary choline levels on the gut microbiota was also characterised in the absence of infection using 16S rRNA gene amplicon sequencing. At 7 d following infection, the levels of C. rodentium in CD mice were significantly greater than that in CS or CE groups (P < 0 center dot 05). CD mice exhibited greater damage to the surface epithelium and goblet cell loss than the CS or CE mice, which was consistent with elevated pro-inflammatory cytokine and chemokine levels in the colon. In addition, CD group exhibited decreased concentrations of PC in the colon after C. rodentium infection, although the decrease was not observed in the absence of challenge. Select genera, including Allobaculum and Turicibacter, were enriched in response to dietary choline deficiency; however, there was minimal impact on the total bacterial abundance or the overall structure of the gut microbiota. Our results suggest that insufficient dietary choline intake aggravates the severity of colitis and demonstrates an essential role of choline in maintaining intestinal homoeostasis.
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