期刊
BRITISH JOURNAL OF DERMATOLOGY
卷 184, 期 6, 页码 1161-1169出版社
WILEY
DOI: 10.1111/bjd.19503
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资金
- NCATS NIH HHS [KL2 TR002492, UL1 TR002494] Funding Source: Medline
- NCI NIH HHS [P30 CA077598] Funding Source: Medline
- EB Charities Funding Source: Medline
- Zona Family Foundation for EB Research Funding Source: Medline
- Richard M. Schulze Family Foundation Funding Source: Medline
This study evaluated the effects of allogeneic epidermal skin grafts on wound healing and durability in patients with RDEB. The results showed significant reductions in wound area, successful fusion of epidermal grafts, and improvement in RDEB pseudosyndactyly.
Background Chronic wounds, a common morbidity in recessive dystrophic epidermolysis bullosa (RDEB), lack definitive therapies. Objectives To assess allogeneic epidermal skin grafts in terms of wound healing and durability over time. Methods In a prospective, open-label clinical trial for postallogeneic haematopoietic cell transplantation (post-alloHCT) patients with RDEB, up to nine chronic wounds per patient were grafted over 1 year. Epidermal grafts measuring 5 cm(2) were obtained from related alloHCT donors in the outpatient setting using the CELLUTOME (TM) Epidermal Harvesting System. Wounds were photographed and symptom inventories completed at baseline and 6, 12 and 52 weeks after grafting. The trial was registered at ClinicalTrials.gov (NCT02670837). Results Between August 2016 and January 2019, eight patients with RDEB received a total of 35 epidermal allografts at a median of 1157 days (range 548-2884) post-alloHCT. The median (interquartile range) percentage reductions in wound surface area were 75% (52-94), 95% (72-100) and 100% (97-100) at 6, 12 and 52 weeks postgraft, respectively, each significantly reduced from baseline (P < 0.001). Donor harvest sites healed quickly without scarring. Biopsy evaluation at 1 year of an epidermal allograft site revealed wildtype type VII collagen (immunofluorescence), anchoring fibrils (electron microscopy) and full-thickness skin whole-DNA donor chimerism of 42% (compared with 16% in concurrently biopsied native skin). This strategy subsequently supported release of RDEB pseudosyndactyly. Conclusions The immune tolerance established by alloHCT supports successful adoptive transfer of donor epidermal grafts. Persistence of donor grafts in a single patient beyond 1 year and observed migration of donor-grafted cells into adjacent wound suggest that epidermal allografts include nonterminally differentiated cells and/or trigger recruitment of donor bone-marrow-derived cells to mediate wound healing.
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