期刊
BIOSENSORS & BIOELECTRONICS
卷 163, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112238
关键词
Tau protein; Dendrimer nanocomposite; Screen-printed electrode; Amperometric sandwich immunoassay; Raw plasma; Brain tissue
类别
资金
- Spanish Ministerio de Economia y Competitividad [CTQ2015-64402-C2-1-R]
- Ministerio de Ciencia, Innovaci.on y Universidades [RTI2018-096135-B-I00]
- TRANSNANOAVANSENS-CM Program from the Comunidad de Madrid [S2018/NMT-4349]
- FAPESP (Sao Paulo Research Foundation (FAPESP)) [2018/14130-7]
- AES-ISCIII program [PI17CIII/00045]
- Fundacion Tatiana Perez de Guzman el Bueno
- FPU predoctoral contract - Spanish Ministerio de Educacion, Cultura y Deporte
This work reports a new sensitive strategy for the determination of tau protein, a hallmark of Alzheimer's disease (AD), involving a sandwich immunoassay and ampemmetric detection at disposable screen-printed carbon electrodes (SPCEs) modified with a gold nanoparticles-poly(amidoamine) (PAMAM) dendrimer nanocomposite (3D-Au-PAMAM) covalently immobilized onto electrografted p-aminobenzoic acid (p-ABA). The capture antibody (CAb) was immobilized by crosslinking with glutaraldehyde (GA) on the amino groups of the 3D-Au-PAMAM-p-ABA-SPCE, where tau protein was sandwiched with a secondary antibody labeled with horseradish peroxidase (HRP-DAb). Amperometry at -200 mV (vs the Ag pseudo-reference electrode) upon the addition of hydroquinone (HQ) as electron transfer mediator and H2O2 as the enzyme substrate was used to detect the immunocomplex formation. The great analytical performance of the immunosensor in terms of selectivity and low limit of detection (LOD) (1.7 pg mL(-1)) allowed the direct determination of the target protein in raw plasma samples and in brain tissue extracts from healthy individuals and post mortem diagnosed AD patients, using a simple and fast protocol.
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