期刊
BIOSENSORS & BIOELECTRONICS
卷 174, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112654
关键词
Dual-ratiometric strategy; Hairpin DNA; Linear ssDNA; Simultaneous determination; Multi-mycotoxins
类别
资金
- National Natural Science Foundation of China [22074055, 62001197, 61901193]
- Innovation/Entrepreneurship Program of Jiangsu Province
- Natural Science Foundation of Jiangsu Province [BK20200104]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD2018-87]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX19_1618]
This work developed a dual-ratiometric electrochemical aptasensing strategy for simultaneous detection of AFB1 and OTA, showcasing a novel way for fabricating a high-performance aptasensor.
The simultaneous detection of multiple mycotoxins in grains is significant due to the enhanced toxicity induced by their synergistic effects. In this work, a dual-ratiometric electrochemical aptasensing strategy for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. Here, an anthraquinone-2-carboxylic acid (AQ)-labelled complementary DNA (cDNA) was used to provide separate and specific binding sites to assemble the ferrocene-labelled AFB1 aptamer (Fc-Apt1) and methylene blue-labelled OTA aptamer (MB-Apt2). The target-induced current ratios of I-Fc/I-AQ and I-MB/I-AQ were then used to quantitatively relate to AFB1 and OTA, respectively. Following this principle, two types of aptasensors involving the hairpin DNA (hDNA) and linear single-stranded DNA (ssDNA) as the cDNA were fabricated for performance comparisons. The results revealed that hairpin DNA with a rigid 2D structure can greatly improve the assembly and recognition efficiency of the sensing interface, which makes the hDNA-based aptasensor possess high sensitivity, reliability and anti-interference ability. The hDNA-based aptasensor exhibited a detection range of 10-3000 pg mL(-1) for AFB1 and 30-10000 pg mL(-1) for OTA, respectively, with no observable cross-reactivity. Furthermore, the aptasensor was applied to analyze corn and wheat samples, and the reliability was validated by HPLC-MS/MS. Our work has presented a novel way for fabricating a high-performance aptasensor for simultaneous detection of multiple mycotoxins.
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