期刊
BIOSENSORS & BIOELECTRONICS
卷 166, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112424
关键词
Colorimetric biosensor; Signal amplification; BRCA1; 2D nanomaterials; Gold nanoparticles; Bismuth selenide nanosheets
类别
资金
- National Natural Science Foundation of China [81872415, 81571764]
Colorimetric biosensors have attracted wide attention due to their low cost, simple operation, rapid response and good reproducibility. However, insufficient sensitivity limits their applications. This report describes the design of a colorimetric biosensor based on a three-step multiple signal amplification strategy to detect breast cancer-associated BRCA1 mutation. The capture unit, signal unit, and target DNA form a sandwich construction. The signal probes are immobilized on the surface of nanomaterials to form the signal unit, which can catalyze the reduction of a colorimetric substrate 4-nitrophenol (4-NP). Firstly, OD gold nanoparticles (AuNPs) are employed to catalyze 4-NP reduction and reaches 10(2)-fold signal amplification. Then AuNPs are decorated on the surface of 2D material, such as graphene oxide (GO), the catalytic efficiency is further enhanced to 10(4)-fold signal amplification. The third step amplification is achieved by replacing stable GO with oxidizable 2D material (Bi2Se3 nanosheets), resulting in a nearly 10(10)-fold amplification. The sandwich-type Bi2Se3-AuNPs biosensor shows excellent sensitivity and selectivity. The detection limit can reach up to 10(-)(18) M and there is a good linear relationship between the reaction kinetics constant and the DNA concentration in the range of 10(-)(12)-10(-)(18) M. In addition, one-base mismatch, two-base mismatch and non-complementary sequences can be distinguished clearly by this biosensor. This design may have beneficial clinical application prospects for cancer genetic screening and early diagnosis.
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