期刊
BIORESOURCE TECHNOLOGY
卷 313, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123646
关键词
Methanotrophs; Microbial protein; Biomethane; Digested biowaste; 16S rRNA
资金
- FUBAF project - Danish EPA - MUDP [Mst-11700508]
To achieve a sustainable production of food and feed production, inexpensive carbon and nutrient sources are needed. In the present study, biologically upgraded biogas is coupled with electrochemically extracted nitrogen from digested biowaste to cultivate mixed methanotrophs as protein source. Results showed that an increase from less than 5 mu gCu(2+)/L to 100 mu gCu(2+)/L increased the biomass production by 41%. Microbial analysis revealed that the dominated Methylomonas spp. followed by Methylophilus spp. created a specialized community for high CH4 assimilation. Moreover, duplicate semi-continuous fermenters run for 120 days validating the efficiency of alternative carbon and nitrogen feedstocks at long-term operation. As for dry cell weight (DCW) production, more than 2.5 g-DCW/L were produced using biologically upgraded biogas and electrochemically extracted nitrogen. Furthermore, the protein content and amino acid pro file (> 50% of DCW) demonstrated that the microbial biomass pose the characteristics to be used as animal feed additive.
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