期刊
BIOPRESERVATION AND BIOBANKING
卷 19, 期 1, 页码 48-52出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/bio.2020.0073
关键词
iFGF23 levels; storage time; repeated freeze-thaw cycles
资金
- National Natural Science Foundation of China [81670699]
The study showed that serum intact FGF23 levels remained stable after storage at -80 degrees Celsius for 6 years. Additionally, undergoing 0-5 freeze-thaw cycles did not significantly affect serum iFGF23 levels.
Background:Fibroblast growth factor 23 (FGF23) has become increasingly important in chronic kidney diseases (CKDs), cardiovascular calcification, and metabolic bone diseases. Fresh or stored blood samples are widely used for the FGF23 assay. Clarifying the factors influencing the FGF23 assay can help to quantify FGF23 more accurately. This study explored the effects of low-temperature storage time and repeated freeze-thaw cycles on the measurement of serum intact FGF23 (iFGF23). Materials and Methods:We selected 60 serum samples from patients with CKD stages 3-5 and hemodialysis patients. An enzyme-linked immunosorbent assay was used to measure the changes in serum iFGF23 levels after 6 years of storage at -80 degrees C. In total, 18 fresh serum samples were frozen and thawed for 0, 1, 3, and 5 cycles to explore the effects of repeated freeze-thaw cycles on serum iFGF23 levels. Results:Median serum iFGF23 concentrations were 252.17 (interquartile range [IQR] 113.82-592.38) pg/mL and 203.85 (IQR 64.76-545.39) pg/mL before and after 6 years. There were no significant differences between them. However, we found a downward trend of 48% in the samples close to the normal level of iFGF23 (<150.34 pg/mL) after 6 years of storage (p = 0.160). In addition, the iFGF23 levels of samples frozen and thawed for 0, 1, 3, and 5 cycles were 278.41 +/- 39.51 (mean +/- standard deviation) pg/mL, 262.84 +/- 38.42 pg/mL, 252.97 +/- 34.65 pg/mL and 250.49 +/- 37.12 pg/mL, respectively. A slight downward trend in iFGF23 levels was observed with increasing freeze-thaw times; however, no significant differences were found among different freeze-thaw cycles. Conclusion:Serum iFGF23 levels remained stable after storage at -80 degrees C for 6 years. In addition, five freeze-thaw cycles had no significant effects on serum iFGF23 levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据