期刊
BIOMATERIALS
卷 253, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2020.120108
关键词
Peripheral neural regeneration; Nerve guidance conduits; Topographical guidance; Anisotropy; Photocatalytic stimulation; Melt electrowriting
资金
- Independent Research Fund Denmark [DFF-7017-00185, DFF-6108-00396]
- Villum Foundation [VKR022954]
- Aarhus University Research Foundation [AUFF-E-2015-FLS-9-18, AUFFE-2015-FLS-7-27]
- European Union [734174]
- Carlsberg Foundation
- Sichuan Science and Technology Foundation [20YYJC3895]
- Fundamental Research Funds for the Central Universities, China [YJ201893]
- State Key Lab of Advanced Metals and Materials, China [2019-Z03]
- Marie Curie Actions (MSCA) [734174] Funding Source: Marie Curie Actions (MSCA)
Great research efforts have been invested in developing nerve guidance conduits (NGCs), which can direct axons advance and guide peripheral neural regeneration. Here, three different aspects of NGC design, namely anisotropy, photocatalytic stimulation and self-assembly at implantation site, were unitedly addressed. Firstly, melt electrowriting (MEW) was used to print anisotropic, microfibrous PCL architectures. Specifically, by tailoring the fiber spacing ratio between two arms of the grid patterns (1-1, 1-2, 1-3), preferential neurite extension of PC 12 cells along the long arm direction was achieved. Such anisotropic neurites guidance was further strengthened when the intersection angles were reduced from 90 degrees to 30 degrees. Secondly, functionalization of PCL micropatterns with graphene oxide and graphitic carbon nitride (g-C3N4), a visible-light photocatalyst, may enable optoelectronic conversion and wireless neural stimulation. As a result, photocatalytic stimulation further enhanced neurite extension length under visible light irradiation. Last but not the least, NGC were successfully obtained either by manually rolling or self-assembly using a thermo-responsive bi-layer system. Interestingly, the anisotropic micropattern design dictated the self-assembly process, and an underlying mechanism was proposed. With a synergy of three unique design parameters, the herein presented NGCs may possess great potential for repairing peripheral nerve injuries.
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