期刊
BIOMATERIALS
卷 252, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2020.120104
关键词
Hydrogels; Growth factors; Fibronectin; poly(ethylene) glycol; Bone; Vascularisation
资金
- UK Regenerative Medicine Platform (MRC) [MR/L022710/1]
- UK Engineering and Physical Sciences Research Council [EPSRC EP/P001114/1]
- Sir Bobby Charlton Foundation
- European Research Council (ERC) under the European Union [615030]
- Biological Services and Veterinary Research Facility (University of Glasagow)
- EPSRC [EP/P001114/1] Funding Source: UKRI
Extracellular matrix (ECM)-derived matrices such as Matrigel are used to culture numerous cell types in vitro as they recapitulate ECM properties that support cell growth, organisation, migration and differentiation. These ECM-derived matrices contain various growth factors which make them highly bioactive. However, they suffer lot-to-lot variability, undefined composition and lack of controlled physical properties. There is a need to develop rationally designed biomaterials that can also recapitulate ECM roles. Here, we report the development of fibronectin (FN)-based 3D hydrogels of controlled stiffness and degradability that incorporate full-length FN to enable solid-phase presentation of growth factors in a physiological manner. We demonstrate, in vitro and in vivo, the effect of incorporating vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP2) in these hydrogels to enhance angiogenesis and bone regeneration, respectively. These hydrogels represent a step-change in the design of well-defined, reproducible, synthetic microenvironments for 3D cell culture that incorporate growth factors to achieve functional effects.
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