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Genetically encoded thiol redox-sensors in the zebrafish model: lessons for embryonic development and regeneration

期刊

BIOLOGICAL CHEMISTRY
卷 402, 期 3, 页码 363-378

出版社

WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2020-0269

关键词

embryo; glutathione; hydrogen peroxide; imaging; regeneration; zebrafish

资金

  1. Schwerpunktprogramm SPP1710 Dynamics of Thiol-based Redox Switches in Cellular Physiologyof the Deutsche Forschungsgemeinschaft [DI913/6-1]
  2. Graduiertenkolleg GRK2039 Molecular Architectures for Fluorescent Imaging of Cells of the Deutsche Forschungsgemeinschaft
  3. Effect-Net research network in water research of the Ministerium fur Wissenschaft, Forschung und Kunst Baden-Wurttemberg
  4. Helmholtz Programme BioInterfaces in Technology and Medicine (BIFTM)

向作者/读者索取更多资源

Zebrafish serves as an excellent model for in vivo bioimaging and possesses remarkable regenerative capacity. Research using genetically encoded redox sensors in this model system has provided important insights into the regulation and downstream effects of redox alterations during tissue development, morphogenesis, and regeneration.
Important roles for reactive oxygen species (ROS) and redox signaling in embryonic development and regenerative processes are increasingly recognized. However, it is difficult to obtain information on spatiotemporal dynamics of ROS production and signaling in vivo. The zebrafish is an excellent model for in vivo bioimaging and possesses a remarkable regenerative capacity upon tissue injury. Here, we review data obtained in this model system with genetically encoded redox-sensors targeting H2O2 and glutathione redox potential. We describe how such observations have prompted insight into regulation and downstream effects of redox alterations during tissue differentiation, morphogenesis and regeneration. We also discuss the properties of the different sensors and their consequences for the interpretation of in vivo imaging results. Finally, we highlight open questions and additional research fields that may benefit from further application of such sensor systems in zebrafish models of development, regeneration and disease.

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