期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1865, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbagen.2020.129771
关键词
MD simulations; HP1; Chromodomain; Peptide recognition; Protein-protein interaction
资金
- Czech Science Foundation [18-07384S]
- Brno PhD Talent Financial Aid
The study shows that the HP1 chromodomain (CD) interacts with its target peptides dynamically, with the target sequence ranging from positions -7 to +3. The CD's anionic clamp can be neutralized by not only the -3 and -1 residues, but also by -7, -6, -5 and +3 residues. Structural dynamics play a significant role in molecular recognition between the HP1 CD domain and its targets, increasing target sequence flexibility through mutual replaceability of target residues.
Background: The chromodomain (CD) of HP1 proteins is an established H3K9(me3) reader that also binds H1, EHMT2 and H3K23 lysine-methylated targets. Structural experiments have provided atomistic pictures of its recognition of the conserved ARK(me3)S/T motif, but structural dynamics' contribution to the recognition may have been masked by ensemble averaging. Methods: We acquired similar to 350 mu s of explicit solvent molecular dynamics (MD) simulations of the CD domain interacting with several peptides using the latest AMBER force fields. Results: The simulations reproduced the experimentally observed static binding patterns well but also revealed visible structural dynamics at the interfaces. While the buried K-0(me3) and A(-2) target residues are tightly bound, several flanking sidechains sample diverse sites on the CD surface. Different amino acid positions of the targets can substitute for each other by forming mutually replaceable interactions with CD, thereby explaining the lack of strict requirement for cationic H3 target residues at the -3 position. The Q(-4) residue of H3 targets further stabilizes the binding. The recognition pattern of the H3K23 ATK(me3)A motif, for which no structure is available, is predicted. Conclusions: The CD reads a longer target segment than previously thought, ranging from positions -7 to +3. The CD anionic clamp can be neutralized not only by the -3 and -1 residues, but also by -7, -6, -5 and +3 residues. General Significance: Structural dynamics, not immediately apparent from the structural data, contribute to molecular recognition between the HP1 CD domain and its targets. Mutual replaceability of target residues increases target sequence flexibility.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据