期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1862, 期 9, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbamem.2020.183359
关键词
GUV; Octanol-assisted liposome assembly; Microfluidics; Lipid composition; FRAP
资金
- Friedrich Naumann Foundation
- Jane Bourque-Driscoll Fund
- Cambridge Philosophical Society
- Engineering and Physical Sciences Research Council (EPSRC)
- Wellcome Trust [204909/Z/16/Z]
- ERC Consolidator Grant [Designer-Pores 647144]
- Winton Programme for the Physics of Sustainability
Giant Unilamellar Vesicles (GUVs) are a versatile tool in many branches of science, including biophysics and synthetic biology. Octanol-Assisted Liposome Assembly (OLA), a recently developed microfluidic technique enables the production and testing of GUVs within a single device under highly controlled experimental conditions. It is therefore gaining significant interest as a platform for use in drug discovery, the production of artificial cells and more generally for controlled studies of the properties of lipid membranes. In this work, we expand the capabilities of the OLA technique by forming GUVs of tunable binary lipid mixtures of DOPC, DOPG and DOPE. Using fluorescence recovery after photobleaching we investigated the lateral diffusion coefficients of lipids in OLA liposomes and found the expected values in the range of 1 mu m(2)/s for the lipid systems tested. We studied the OLA derived GUVs under a range of conditions and compared the results with electroformed vesicles. Overall, we found the lateral diffusion coefficients of lipids in vesicles obtained with OLA to be quantitatively similar to those in vesicles obtained via traditional electroformation. Our results provide a quantitative biophysical validation of the quality of OLA derived GUVs, which will facilitate the wider use of this versatile platform.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据