期刊
ASTROBIOLOGY
卷 21, 期 2, 页码 191-198出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ast.2020.2263
关键词
Peroxide; Catalase; Biosensor; mu PAD
资金
- Clemson University
Hydrogen peroxide may be present on the surface of Europa and Enceladus, potentially serving as an energy source for life-forms but also interfering with current detection technologies. In order to address this issue, researchers have immobilized catalase onto silica particles and used it in combination with a microfluidic paper-based analytical device for selective sample pretreatment.
Hydrogen peroxide has been postulated to be present on the surface of Europa and Enceladus. While it could represent a potential source of energy for possible life-forms, H(2)O(2)may also interfere with a number of current detection technologies, including biosensors. To take advantage of the selectivity and portability of these devices, simple and reliable routes to degrade the potential H(2)O(2)present should be developed and implemented to prepare for this possibility. Unfortunately, most of the current approaches for removing H(2)O(2)are slow, may affect the sample, or could interfere with the performance of biosensors. To address these limitations, catalase was immobilized onto silica particles and used as a means to selectively decompose H(2)O(2)prior to the analysis of common biomarkers with a biosensor. For these experiments, glucose,l-leucine, and lactic acid were used as representative examples of biomolecules such as carbohydrates, amino acids, and organic acids, respectively, which could be used as biomarkers on extraterrestrial bodies. While the decomposition reaction between catalase and H(2)O(2)is well known, to our knowledge this is the first instance where catalase has been used in combination with a microfluidic paper-based analytical device (mu PAD) to implement selective sample pretreatment.
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