期刊
ARCHIVES OF VIROLOGY
卷 165, 期 12, 页码 3017-3021出版社
SPRINGER WIEN
DOI: 10.1007/s00705-020-04838-6
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资金
- Japan Society for the Promotion of Science (JSPS)
- Directorate General of Resources for Science Technology and Higher Education (DG-RSTHE) under the JSPS-DG-RSTHE Joint Research Program
- JSPS KAKENHI Grant [19H02950]
- Grants-in-Aid for Scientific Research [19H02950] Funding Source: KAKEN
In 2017, a leaf sample from a single chili pepper (Capsicum annuum) plant exhibiting yellowing was collected from Aceh province, Indonesia. Total RNA was extracted from this sample, and RNA-Seq analysis was conducted. Putative infecting viruses were detected by mapping the obtained reads to the full-length viral genome sequences available in the GenBank database (7457 sequences) and the de novo-assembled contigs. RNA-Seq analysis detected polerovirus, begomovirus, and amalgavirus sequences, and the polerovirus-like sequences showed strong similarity to those of previously reported pepper vein yellows viruses (PeVYVs). The complete viral genome sequence obtained by RT-PCR had a length of 6023 nt, had the typical genome organization of a polerovirus and showed a high degree of sequence similarity to PeVYV-2 from Israel. Moreover, the predicted amino acid sequence of the P0 protein of the Indonesian isolate was 85.1% to 88.8% identical to those of other PeVYVs. In accordance with the polerovirus species demarcation criteria, this isolate should be assigned to a new polerovirus species, and we propose the name pepper vein yellows virus 9 (PeVYV-9) for this virus.
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