期刊
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
卷 105, 期 4, 页码 -出版社
WILEY
DOI: 10.1002/arch.21739
关键词
double-stranded RNA; insecticidal activity; Laodelphax striatellus; RNA interference
资金
- Rural Development Administration [PJ01497803]
The small brown planthopper,Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to controlL. striatellusfor more than a decade, the occurrence of buprofezin-resistantL. striatellus has been recently reported. To develop an alternative pest control strategy, comparative transcriptome analysis of buprofezin-treated and nontreatedL. striatelluswas performed to screen the buprofezin-specific target genes for RNA interference (RNAi) application. Among six genes downregulated in the buprofezin-treatedL. striatellus, RNAi-based silencing of the lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase significantly induced the lethality ofL. striatellusin a concentration-dependent manner. In addition, a cocktail of double-stranded RNAs against these three genes showed synergistic effects with buprofezin. These results provide RNAi-based effective approaches to controlL. striatellusas well as an efficient method to identify novel target genes for RNAi application.
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