期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 51, 页码 23025-23029出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202009800
关键词
EPR spectroscopy; molecular dynamics; PELDOR/DEER spectroscopy; RNA structures; site-directed spin labeling
资金
- German Research Foundation [CRC902]
- Icelandic Research Fund [141062051]
- Max Planck Society
- Human Frontier Science Program [RGP0026/2017]
- Projekt DEAL
The structure and flexibility of RNA depends sensitively on the microenvironment. Using pulsed electron-electron double-resonance (PELDOR)/double electron-electron resonance (DEER) spectroscopy combined with advanced labeling techniques, we show that the structure of double-stranded RNA (dsRNA) changes upon internalization intoXenopus laevis oocytes. Compared to dilute solution, the dsRNA A-helix is more compact in cells. We recapitulate this compaction in a densely crowded protein solution. Atomic-resolution molecular dynamics simulations of dsRNA semi-quantitatively capture the compaction, and identify non-specific electrostatic interactions between proteins and dsRNA as a possible driver of this effect.
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