期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 6, 页码 3121-3130出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202011711
关键词
ATP synthesis; cerium vanadate; nanozymes; oxidative stress; superoxide dismutase
资金
- Science and Engineering Research Board (SERB), New Delhi [EMR/IISc-01/2016]
- DST Nanomission, New Delhi [SR/NM/NS-1380/2014]
- DST [SB/S2/JCB-067/2015]
- IISc
- SERB
The study demonstrates that CeVO4 nanozyme can substitute the function of SOD1 and SOD2 in neuronal cells even when the natural enzymes are down-regulated. The nanozyme prevents mitochondrial damage by regulating superoxide levels and restores physiological levels of anti-apoptotic proteins. Additionally, it effectively prevents mitochondrial depolarization and improves cellular ATP levels under oxidative stress.
Nanoparticles that functionally mimic the activity of metal-containing enzymes (metallo-nanozymes) are of therapeutic importance for treating various diseases. However, it is still not clear whether such nanozymes can completely substitute the function of natural enzymes in living cells. In this work, we show for the first time that a cerium vanadate (CeVO4) nanozyme can substitute the function of superoxide dismutase 1 and 2 (SOD1 and SOD2) in the neuronal cells even when the natural enzyme is down-regulated by specific gene silencing. The nanozyme prevents the mitochondrial damage in SOD1- and SOD2-depleted cells by regulating the superoxide levels and restores the physiological levels of the anti-apoptotic Bcl-2 family proteins. Furthermore, the nanozyme effectively prevents the mitochondrial depolarization, leading to a significant improvement in the cellular levels of ATP under oxidative stress.
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