期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 50, 页码 22456-22459出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202008067
关键词
biocatalysis; chemoenzymatic synthesis; glycans; oligosaccharides; transgalactosylation
资金
- Marie Sklodowska-Curie Innovative Training Network [Marie-Curie ITN GLYCOVAX] [675671]
Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks. Herein we demonstrate the use of biocatalysis to supply such reagents for automated synthesis. By using the promiscuous NmLgtB-B beta 1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors. The enzymatic product was shown to be successfully incorporated as a complete unit into a tetrasaccharide target by automated assembly.
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