期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 412, 期 29, 页码 8117-8126出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-02942-9
关键词
Ultra-sensitive; Plasmon-coupled microcavities; SERS; Pathogens; Dual-mode; Fluorescence
资金
- NSFC [11734005, 61821002]
- National Key R&D Program of China [2017YFA0700500, 2018YFA0209101]
Septicemia and bacteremia are serious infections in the bloodstream. Thus, time-saving and ultra-sensitive pathogenic bacteria detection is highly required. Herein, we constructed gold nanoparticle-modified polystyrene microspheres (Au/PS) as plasmon-coupled microcavities to realize simultaneous detection ofStaphylococcus aureusandEscherichia colibased on a fluorescence and surface-enhanced Raman spectroscopy (SERS) dual-mode method. Fluorescence imaging, serving as a means for assistant validation and rapid screening, was carried out to achieve qualitative and semi-quantitative determination, which gave us visual information of the existence and distribution of the target bacteria. Meanwhile, SERS test was conducted to realize ultra-sensitive quantitative detection. The evanescent wave aroused from total internal reflection in PS microcavities coupled with the localized electromagnetic field from surface plasmons of gold nanoparticles to improve light-matter interaction synergistically, leading to an enhancement factor of 2.25 x 10(11)for SERS sensing. The whole measurement was carried out in a typical sandwich assay of capture probe-target bacteria-signal probe. As a result, calibrated concentration response curves demonstrated the sensitive quantitative detection with the limit of detection (LOD) of 3 cfu/mL forS. aureusand 2 cfu/mL forE. coli. This rapid, ultra-sensitive, and visual sensing method was further developed for dual-bacteria detection in the whole blood samples.
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