期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 412, 期 28, 页码 7819-7826出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-02911-2
关键词
Fluorescence; Fluorescent probe; Viscosity; Biosensors; UV/VIS
资金
- National Natural Science Foundation of China [51703171, 51873160]
- Science Foundation of Wuhan Institute of Technology [K201754]
Intracellular viscosity is closely related to a series of biological processes and could be a biomarker for various diseases. Herein, we reported a deep-red emission viscosity probeACI, which showed a turn-on fluorescence effect with excellent selectivity encountering high viscous medium. To assure the practical biological application,ACIdemonstrated not only a long wavelength emission at 634 nm but also a long wavelength excitation at 566 nm, which were crucial to afford deeper penetration depth and higher sensitivity in bioimaging. The photophysical properties and viscosity recognition mechanism of the probe were carefully discussed here. Theoretical calculations furtherly confirmed that high viscous medium could inhibit the twisted intramolecular charge transfer (TICT) process of the probe which quenched the fluorescence in low viscous media, and restore the emission. More importantly, it was successfully applied to visualize the viscosity in living cells.
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