期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 413, 期 1, 页码 73-82出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-02953-6
关键词
Biosensors; Heavy metals; Sensing module; Reporter module; Metal-induced transcription
Whole-cell heavy metal biosensing based on optical transduction has made significant advancements, improving sensor performance and introducing new challenges. Genetic modulation in recombinant plasmids and the utilization of novel platforms like microfluidics have shown promising results for the field of optical biosensing.
A large number of scientific works have been published on whole-cell heavy metal biosensing based on optical transduction. The advances in the application of biotechnological tools not only have continuously improved the sensitivity, selectivity, and detection range for biosensors but also have simultaneously unveiled new challenges and restrictions for further improvements. This review highlights selected aspects of whole-cell biosensing of heavy metals using optical transducers. We have focused on the progress in genetic modulation in regulatory and reporter modules of recombinant plasmids that has enabled improvement of biosensor performance. Simultaneously, an attempt has been made to present newer platforms such as microfluidics that have generated promising results and might give a new turn to the optical biosensing field.
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