期刊
ACS NANO
卷 14, 期 10, 页码 13451-13462出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.0c05340
关键词
DNA nanotechnology; DNA origami; DNA strand displacement; self-assembly; dynamic nanostructures; nucleic acid diagnostics
类别
资金
- National Science Foundation Graduate Research Fellowship [DGE-1232825]
- Department of Energy [DESC001 0426]
Molecular assemblies inside cells often undergo structural reconfiguration in response to stimuli to alter their function. Adaptive reconfiguration of cytoskeletal networks, for example, enables cellular shape change, movement, and cargo transport and plays a key role in driving complex processes such as division and differentiation. The cellular cytoskeleton is a self-assembling polymer network composed of simple filaments, so reconfiguration often occurs through the rearrangement lof its component filaments' connectivities. DNA nanotubes have emerged as promising building blocks for constructing programmable synthetic analogs of cytoskeletal networks. Nucleating seeds can control when and where nanotubes grow, and capping structures can bind nanotube ends to stop growth. Such seeding and capping structures, collectively called termini,can organize nanotubes into larger architectures. However, these structures cannot be selectively activated or inactivated in response to specific stimuli to rearrange nanotube architectures, a key property of cytoskeletal networks. Here, we demonstrate how selective lregulation of the binding affinity of DNA nanotube termini lfor DNA nanotube monomers or nanotube ends lcan direct the reconfiguration of nanotube architectures. Using DNA hybridization and strand displacement reactions that specifically activate or inactivate four orthogonal nanotube termini, we demonstrate that nanotube architectures bereconfigured by, selective addition or removal of distinct termini. Finally, we show how terminus activation could be can a sensitive detector and l amplifier of a DNA sequence signal. These results could enable the development of adaptive and multifunctional materials or diagnostic tools.
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