期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 36, 页码 39991-40001出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c08784
关键词
wound dressing; cellulose; antibacterial activity; biocompatibility; epsilon-poly-l-lysine
资金
- Canada Research Chairs program
- Canada Foundation for Innovation
- Natural Sciences and Engineering Research Council of Canada (NSERC) [2018-05781]
- NSERC-FPInnovation's Strategic Research Project [506303-17]
- McGill University for a MEDA fellowship
- Natural Sciences and Engineering Research Council (NSERC) [RGPIN-06671-14]
- Townshend-Lamarre Family Foundation
- McGill University
In this study, a carboxyl-modified cellulosic hydrogel was developed as the base material for wound dressings. epsilon-polyL-lysine, a natural polyamide, was then covalently linked to the hydrogel through a bioconjugation reaction, which was confirmed by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR). The antibacterial efficacy of the hydrogel was tested against two model bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, two of the most commonly found bacteria in wound infections. Bacterial viability and biofilm formation after exposure of bacteria to the hydrogels were used as efficacy indicators. Live/Dead assay was used to measure the number of compromised bacteria using a confocal laser scanning microscope. The results show that the antibacterial hydrogel was able to kill approximately 99% of the exposed bacteria after 3 h of exposure. In addition, NIH/3T3 fibroblasts were used to study the biocompatibility of the developed hydrogels. Water-soluble tetrazolium salt (WST)-1 assay was used to measure the metabolic activity of the cells and Live/Dead assay was used to measure the viability of the cells after 24, 48, and 72 h. The developed antibacterial hydrogels are light weight, have a high water-uptake capacity, and show high biocompatibility with the model mammalian cells, which make them a promising candidate to be used for wound dressing applications.
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