期刊
PLANT DIRECT
卷 4, 期 5, 页码 -出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/pld3.219
关键词
Arabidopsis thaliana; hydroxyl carlactone derivative; lateral branching oxidoreductase
资金
- Japan Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry
- Japan Society for the Promotion of Sciences [15K07093, 16K07618, 16K18560, 17K07650]
- Japan Science and Technology Agency [JPMJPR17QA]
- Australian Research Council [DP110100808, FT180100081]
Strigolactones (SLs) regulate important aspects of plant growth and stress responses. Many diverse types of SL occur in plants, but a complete picture of biosynthesis remains unclear. In Arabidopsis thaliana, we have demonstrated that MAX1, a cytochrome P450 monooxygenase, converts carlactone (CL) into carlactonoic acid (CLA) and that LBO, a 2-oxoglutarate-dependent dioxygenase, can convert methyl carlactonoate (MeCLA) into a metabolite called [MeCLA + 16 Da]. In the present study, feeding experiments with deuterated MeCLAs revealed that [MeCLA + 16 Da] is hydroxymethyl carlactonoate (1'-HO-MeCLA). Importantly, this LBO metabolite was detected in plants. Interestingly, other related compounds, methyl 4-hydroxycarlactonoate (4-HO-MeCLA) and methyl 16-hydroxycarlactonoate (16-HO-MeCLA), were also found to accumulate in lbo mutants. 3-HO-, 4-HO-, and 16-HO-CL were detected in plants, but their expected corresponding metabolites, HO-CLAs, were absent in max1 mutants. These results suggest that HO-CL derivatives may be predominant SLs in Arabidopsis, produced through MAX1 and LBO.
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