期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷 187, 期 -, 页码 74-80出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2016.05.008
关键词
Endocrine disruption; Gonad culture; Organochlorine pesticide; Commercial formulation; Active ingredients; Endosulfan
资金
- Universidad de Buenos Aires [UBACyT x650]
- CONICET [PIP 1021]
- CONICET-FAPESP
The organochlorine pesticide endosulfan (ES) is used in several countries as a wide spectrum insecticide on crops with high commercial value. Due to its high toxicity to non -target animals, its persistence in the environment and its ability to act as an endocrine disrupting compound in fish, ES use is currently banned or restricted in many other countries. Previous studies on the cichlid fish Cichlasoma dimerus have shown that waterborne exposure to ES can lead to both decreased pituitary FSH content and histological alterations of testes. As gonadotropin-stimulated sex steroids release from gonads was inhibited by ES in vitro, the aim of the present study was to elucidate possible mechanisms of disruption of ES on gonadal steroidogenesis in C. dimerus, as well as compare the action of the active ingredient (AI) with that of currently used commercial formulations (CF). Testis and ovary fragments were incubated with ES (AI or CF) and/or steroidogenesis activators or precursors. Testosterone and estradiol levels were measured in the incubation media. By itself, ES did not affect hormone levels. Co-incubation with LH and the adenylate cyclase activator forskolin caused a decrease of the stimulated sex steroids release. When co-incubated with precursors dehydroandrostenedione and 17 alpha hydroxyprogesterone, ES did not affect the increase caused by their addition alone. No differences were observed between the Al and CFs, suggesting that the effect on steroidogenesis disruption is mainly caused by the Al. Results indicate that action of ES takes place downstream of LH-receptor activation and upstream of the studied steroidogenic enzymes. (C) 2016 Elsevier Inc. All rights reserved.
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