Behavioral and biochemical adjustments of the zebrafish Danio rerio exposed to the β-blocker propranolol

Propranolol (PROP) is a beta-blocicer prescribed mainly to treat human cardiovascular diseases and as a result of its wide usage and persistence, it is reported in aquatic environments. This study examined whether PROP alters developmental patterns and catecholamine (CA)-regulated processes in the zebrafish (Danio redo) and if exposure during early life alters the stress response and behaviors of adults. The calculated 48 h larva LC50 was 21.6 mg/L, well above reported environmental levels (0.01-0.59 mu g/L). Stressed and PROP-exposed adult zebrafish had reduced testosterone and estradiol levels and exhibited behaviors indicating less anxiety than control fish. Furthermore, adults previously PROP-exposed as embryos/larvae had decreased growth in terms of body length and mass. Finally, these adults showed increased cholesterol and a dose-dependent decrease in testosterone levels compared with unexposed zebrafish. Thus PROP-exposure of zebrafish embryos/larvae alters developmental patterns and CA -regulated processes that may affect normal behaviors and responses to stressors, and at least some of these changes persist in the adult zebrafish. (C) 2015 Elsevier Inc. All rights reserved.