期刊
MEMBRANES
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/membranes10050090
关键词
electrodialysis; ultrafiltration membrane; antimicrobial peptide; waste valorisation
类别
资金
- Bio Based Industries Joint Undertaking under European Union's Horizon 2020 research and innovation program [745762]
- H2020 Societal Challenges Programme [745762] Funding Source: H2020 Societal Challenges Programme
The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, alpha 137-141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the alpha 137-141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase-75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the alpha 137-141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF alpha 137-141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.
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