期刊
ISCIENCE
卷 23, 期 6, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2020.101151
关键词
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资金
- National Institutes of Health (NIH) [GM117942]
- American Heart Association [17PRE33410952]
- UCI School of Medicine Behrens Research Excellence Award
- NIH [AG053592, DK114652]
- Novo Nordisk Foundation Challenge Grant
- Institut National de la Sante et de la Recherche Medicale (U1233 INSERM, France)
The transcription factor BMAL1 is a core element of the circadian clock that contributes to cyclic control of genes transcribed by RNA polymerase II. By using biochemical cellular fractionation and immunofluorescence analyses we reveal a previously uncharacterized nucleolar localization for BMAL1. We used an unbiased approach to determine the BMAL1 interactome by mass spectrometry and identified NOP58 as a prominent nucleolar interactor. NOP58, a core component of the box C/D small nucleolar ribonucleoprotein complex, associates with Snord118 to control specific pre-ribosomal RNA (pre-rRNA) processing steps. These results suggest a non- canonical role of BMAL1 in ribosomal RNA regulation. Indeed, we show that BMAL1 controls NOP58-associated Snord118 nucleolar levels and cleavage of unique pre-rRNA intermediates. Our findings identify an unsuspected function of BMAL1 in the nucleolus that appears distinct from its canonical role in the circadian clock system.
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