期刊
SMALL METHODS
卷 4, 期 11, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smtd.202000101
关键词
circulating-tumor DNA; liquid biopsy; nanopore sensing; single-point mutation
资金
- KAKENHI [17K19138, 19H00901]
- MEXT, Japan
- Grants-in-Aid for Scientific Research [19H00901, 17K19138] Funding Source: KAKEN
Here the recognition of a single-point mutation in oligonucleotides is described by using nanopore measurements. The translocation behavior of a series of mutated DNA strands, hybridized with a complementary DNA probe, is analyzed via blocking current and unzipping time. Discernment of the mutation position at the single nucleotide level is achieved by analysis of a 2D graph of the bootstrapped translocation data. The proposed approach provides a useful tool for the mutation detection of oligonucleotides secreted from tumor cells and is applicable in simple and label-free diagnoses as a nanopore liquid biopsy.
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