期刊
SMALL METHODS
卷 4, 期 11, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smtd.201900893
关键词
electroosmotic flow; translocation events; nanopores; proteins
资金
- National Key Research and Development Program of China [2018YFB1105400]
- Natural Science Foundation of Jiangsu Province [BK20180400, BK20190358]
- Natural Science Foundation of China [51605090, 51905097, 51675101]
- Fundamental Research Funds for the Central Universities [2242019K40272, 2242019k1G011]
- Open Foundation of Key Laboratory for Micro/Nano Technology and System of Liaoning Province
Solid-state nanopores can be a powerful tool to investigate proteins in their native state. However, the excessive fast translocation speed of proteins causes the majority of events to remain undetected. Here, the behaviors of streptavidin (SA) transport through a solid-state nanopore are dominated by electroosmotic flow (EOF). Experimental results show the frequency of translocation events detected by the nanopore with a diameter slightly larger than length is approximate to 17 times larger than that in previously reported work. Numerical simulations elucidate the enhanced frequency comes from a concave-shape EOF in the nanopore, which provides a low water velocity region allowing for numbers of SAs moving slowly enough to be detected. However, as pore diameter increases, the maximum detectable velocity of SAs decreases due to the reduced signal-to-noise ratio. Moreover, it is found the SA translocation frequency slowly increases with the amplitude of voltages, and then declines at a threshold bias. This phenomenon can be attributed to the bandwidth limitation combined with the non-linear dependence of EOF velocity in the pore central region on applied voltage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据