期刊
METABOLITES
卷 10, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/metabo10060250
关键词
cyanobacteria; metabolomics; CRISPR-Cpf1; C-13 isotopic labelling; ethylene; succinate
资金
- Department of Biotechnology, Government of India [BT/EB/PAN IIT/2012]
- Indo-US Science and Technology Forum for Indo-US Advanced Bioenergy Consortium (IUABC) [IUSSTF/JCERDC-SGB/IUABC-IITB/2016]
- O ffice of Science, US Department of Energy-BER [DE-SC0019386]
- Praj Industries Ltd.
- Praj Industries Ltd., India
- U.S. Department of Energy (DOE) [DE-SC0019386] Funding Source: U.S. Department of Energy (DOE)
Cyanobacteria are emerging as hosts for photoautotrophic production of chemicals. Recent studies have attempted to stretch the limits of photosynthetic production, typically focusing on one product at a time, possibly to minimise the additional burden of product separation. Here, we explore the simultaneous production of two products that can be easily separated: ethylene, a gaseous product, and succinate, an organic acid that accumulates in the culture medium. This was achieved by expressing a single copy of the ethylene forming enzyme (efe) under the control of P-cpcB, the inducer-free super-strong promoter of phycocyanin beta subunit. We chose the recently reported, fast-growing and robust cyanobacterium,Synechococcus elongatus PCC 11801, as the host strain. A stable recombinant strain was constructed using CRISPR-Cpf1 in a first report of markerless genome editing of this cyanobacterium. Under photoautotrophic conditions, the recombinant strain shows specific productivities of 338.26 and 1044.18 mu mole/g dry cell weight/h for ethylene and succinate, respectively. These results compare favourably with the reported productivities for individual products in cyanobacteria that are highly engineered. Metabolome profiling and C-13 labelling studies indicate carbon flux redistribution and suggest avenues for further improvement. Our results show that S. elongatus PCC 11801 is a promising candidate for metabolic engineering.
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