期刊
PATHOGENS
卷 9, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/pathogens9070545
关键词
Mycoplasma bovis; minimum inhibitory concentration; antimicrobial resistance; mutations; Spain
类别
资金
- Spanish Ministry of Economy and Competitiveness (Spanish Government) - FEDER funds [AGL2016-76568-R]
- State Subprogram Training of the State Program for the Promotion of Talent and its Employability [BES-2017-080186]
Mycoplasma bovisis an important bovine pathogen causing pneumonia, mastitis, and arthritis and is responsible for major economic losses worldwide. In the absence of an efficient vaccine, control ofM. bovisinfections mainly relies on antimicrobial treatments, but resistance is reported in an increasing number of countries. To address the situation in Spain,M. boviswas searched in 436 samples collected from beef and dairy cattle (2016-2019) and 28% were positive. Single-locus typing usingpolCsequences further revealed that two subtypes ST2 and ST3, circulate in Spain both in beef and dairy cattle, regardless of the regions or the clinical signs. Monitoring of ST2 and ST3 isolates minimum inhibitory concentration (MIC) to a panel of antimicrobials revealed one major difference when using fluoroquinolones (FQL): ST2 is more susceptible than ST3. Accordingly, whole-genome sequencing (WGS) further identified mutations in thegyrAandparCregions, encoding quinolone resistance-determining regions (QRDR) only in ST3 isolates. This situation shows the capacity of ST3 to accumulate mutations in QRDR and might reflect the selective pressure imposed by the extensive use of these antimicrobials. MIC values and detection of mutations by WGS also showed that most Spanish isolates are resistant to macrolides, lincosamides, and tetracyclines. Valnemulin was the only one effective, at least in vitro, against both STs.
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