期刊
ANTIOXIDANTS
卷 9, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/antiox9070627
关键词
diabetic retinopathy; db/db mice; Cinnamomi Ramulus; Paeoniae Radix; CPA4-1; blood-retinal barrier; occludin
资金
- Korea Institute of Oriental Medicine [K18270, KSN20134261]
- National Research Council of Science & Technology (NST), Republic of Korea [K18270] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Increased formation of advanced glycation end products (AGEs) plays an important role in the development of diabetic retinopathy (DR) via blood-retinal barrier (BRB) dysfunction, and reduction of AGEs has been suggested as a therapeutic target for DR. In this study, we examined whether CPA4-1, a herbal combination of Cinnamomi Ramulus and Paeoniae Radix, inhibits AGE formation. CPA4-1 and fenofibrate were tested to ameliorate changes in retinal capillaries and retinal occludin expression in db/db mice, a mouse model of obesity-induced type 2 diabetes. CPA4-1 (100 mg/kg) or fenofibrate (100 mg/kg) were orally administered once a day for 12 weeks. CPA4-1 (the half maximal inhibitory concentration, IC50=6.84 +/- 0.08 mu g/mL) showed approximately 11.44-fold higher inhibitory effect on AGE formation than that of aminoguanidine (AG, the inhibitor of AGEs, IC50= 78.28 +/- 4.24 mu g/mL), as well as breaking effect on AGE-bovine serum albumin crosslinking with collagen (IC50= 1.30 +/- 0.37 mu g/mL). CPA4-1 treatment ameliorated BRB leakage and tended to increase retinal occludin expression in db/db mice. CPA4-1 or fenofibrate treatment significantly reduced retinal acellular capillary formation in db/db mice. These findings suggested the potential of CPA4-1 as a therapeutic supplement for protection against retinal vascular permeability diseases.
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