期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2020.00852
关键词
carbon catabolite repression; transcription factor; Cre1; cellulase; phosphorylation; Trichoderma reesei
资金
- National Key R&D Program of China [2018YFB1501701]
- National Natural Science Foundation of China [31570040, 31870785]
- Key Technologies R&D Program of Shandong Province [2018GSF121021]
- State Key Laboratory of Microbial Technology Open Projects Fund
InTrichoderma reesei, carbon catabolite repression (CCR) significantly downregulates the transcription of cellulolytic enzymes, which is usually mediated by the zinc finger protein Cre1. It was found that there is a conserved region at the C-terminus of Cre1/CreA in several cellulase-producing fungi that contains up to three continuous S/T phosphorylation sites. Here, S387, S388, T389, and T390 at the C-terminus of Cre1 inT. reeseiwere mutated to valine for mimicking an unphosphorylated state, thereby generating the transformantsTr_Cre1(S387V),Tr_Cre1(S388V),Tr_Cre1(T389V), andTr_Cre1(T390V), respectively. Transcription ofcel7ainTr_ Cre1(S388V)was markedly higher than that of the parent strain when grown in glucose-containing media. Under these conditions, both filter paperase (FPase) andp-nitrophenyl-beta-(D)-cellobioside (pNPCase) activities, as well as soluble proteins fromTr_Cre1(S388V)were significantly increased by up to 2- to 3-fold compared with that of other transformants and the parent strain. The results suggested that S388 is critical site of phosphorylation for triggering CCR at the terminus of Cre1. To our knowledge, this is the first report demonstrating an improvement of cellulase production inT. reeseiunder CCR by mimicking dephosphorylation at the C-terminus of Cre1. Taken together, we developed a precision engineering strategy based on the modification of phosphorylation sites of Cre1 transcription factor to enhance the production of cellulase inT. reeseiunder CCR.
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