期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2020.00616
关键词
three-dimensional scaffolds; biomaterials; polydopamine; carbon microfiber; neural stem cell
资金
- National Key R&D Program of China [2017YFA0103903]
- Strategic Priority Research Program of the Chinese Academy of Science [XDA16010303]
- National Natural Science Foundation of China [81970882]
- 111 Project of the Ministry of Education of China [B17011]
- Natural Science Foundation from Jiangsu Province [BE2019711]
- Boehringer Ingelheim Pharma GmbH
- K. C. Wong Education Foundation
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX19_0057]
Neuroregenerative medicine has witnessed impressive technological breakthroughs in recent years, but the currently available scaffold materials still have limitations regarding the development of effective treatment strategies for neurological diseases. Electrically conductive micropatterned materials have gained popularity in recent years due to their significant effects on neural stem cell fate. Polydopamine (PDA)-modified materials can also enhance the differentiation of neurons. In this work, we show that PDA-modified carbon microfiber skeleton composites have the appropriate conductivity, three-dimensional structure, and microenvironment regulation that are crucial for the growth of neural stem cells. The design we present is low-cost and easy to make and shows great promise for studying the growth and development of mouse neural stem cells. Our results show that the PDA-mediated formation of electrically conductive and viscous nanofiber webs promoted the adhesion, organization, and intercellular coupling of neural stem cells relative to the control group. PDA induced massive proliferation of neural stem cells and promoted the expression of Ki-67. Together, our results suggest that the composite material can be used as a multifunctional neural scaffold for clinical treatment andin vitroresearch by improving the structure, conductivity, and mechanical integrity of the regenerated tissues.
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