期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2020.00567
关键词
urinary tract development; kidney formation; zebrafish development; cloacal malformation; functional genetics; CAKUT; bladder exstrophy-epispadias complex
资金
- BONFOR program of the University of Bonn [O-149.0112]
- German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) [RE 1723/1-1, TH 1327/1-1]
- DFG [RE 1723/1-3, OD 102/13, INST 1172/37-1 FUGG]
- BONFOR [O-149.0123]
- Medical Research Council [MR/L002744/1]
- Horizon 2020 Marie Sklodowska-Curie Actions Initial Training Network [942937]
- University of Manchester Strategic Fund
- Wellcome Trust
- Bonn Medical Faculty zebrafish core facility
- MRC [MR/R006237/1] Funding Source: UKRI
Previous studies in developingXenopusand zebrafish reported that the phosphate transporterslc20a1ais expressed in pronephric kidneys. The recent identification ofSLC20A1as a monoallelic candidate gene for cloacal exstrophy further suggests its involvement in the urinary tract and urorectal development. However, little is known of the functional role ofSLC20A1in urinary tract development. Here, we investigated this using morpholino oligonucleotide knockdown of the zebrafish orthologslc20a1a. This caused kidney cysts and malformations of the cloaca. Moreover, in morphants we demonstrated dysfunctional voiding and hindgut opening defects mimicking imperforate anus in human cloacal exstrophy. Furthermore, we performed immunohistochemistry of an unaffected 6-week-old human embryo and detectedSLC20A1in the urinary tract and the abdominal midline, structures implicated in the pathogenesis of cloacal exstrophy. Additionally, we resequencedSLC20A1in 690 individuals with bladder exstrophy-epispadias complex (BEEC) including 84 individuals with cloacal exstrophy. We identified two additional monoallelicde novovariants. One was identified in a case-parent trio with classic bladder exstrophy, and one additional novelde novovariant was detected in an affected mother who transmitted this variant to her affected son. To study the potential cellular impact ofSLC20A1variants, we expressed them in HEK293 cells. Here, phosphate transport was not compromised, suggesting that it is not a disease mechanism. However, there was a tendency for lower levels of cleaved caspase-3, perhaps implicating apoptosis pathways in the disease. Our results suggestSLC20A1is involved in urinary tract and urorectal development and implicateSLC20A1as a disease-gene for BEEC.
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