期刊
BIOMASS CONVERSION AND BIOREFINERY
卷 12, 期 6, 页码 2133-2147出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13399-020-00798-8
关键词
Acetate; pH; Fatty acid methyl esters (FAME); Microalgae; Surface response methodology
资金
- Research Fund of the Istanbul Technical University [40564]
This study aimed to optimize the growth conditions of microalgae Auxenochlorella protothecoides to maximize biomass and lipid content. By varying pH values and acetate concentrations in the culture medium, the researchers identified the optimal conditions that resulted in the highest biomass and lipid content. Microalgae growth under these optimized conditions can enhance biomass and lipid productivity, providing suitable conditions for the production of high-quality biodiesel.
This study aimed to optimize the growth conditions of the microalgaeAuxenochlorella protothecoidesto maximize the biomass and lipid content. The pH values and acetate concentrations of a tris-acetate-phosphate medium were varied for a photoheterotrophically grown culture ofA. protothecoides. Approximately 23% of the microalgal biomass consisted of saturated fatty acids (SFAs). The highest lipid content (47.8%) was achieved with a medium containing 17 mM acetate at pH 6.2, and a medium containing 50 mM acetate at pH 5.8 resulted in the highest SFA content (43.7%). Surface response methodology was used to analyze the effects of two independent variables, pH (5.8-7.2) and acetate (0-35 mM), on lipid and biomass content. Four quadratic models, using the Box-Wilson central composite design, were proposed to correlate the optimal pH and acetate concentrations that would achieve the maximum biomass and lipid content. The study demonstrated that medium containing 24.4 mM acetate at pH 5.96 resulted in the highest lipid content (47.8%), and resulted in the highest biomass (1537 mg/L). Microalgae growth under these optimized conditions can enhance biomass and lipid productivity and can provide suitable conditions for high-quality biodiesel.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据