期刊
FOOD SCIENCE & NUTRITION
卷 8, 期 7, 页码 3947-3956出版社
WILEY
DOI: 10.1002/fsn3.1710
关键词
amino acid composition; anti-inflammation ability; antioxidant activities; bioactive peptide fractions; oyster soft tissue hydrolysates
资金
- Qing Lan Project
- Jiangsu Provincial Population and Family Planning Commission [J201605]
- Yancheng Medical Science and Technology Development Project [YK2016045]
- Project of Jiangsu Provincial Department of Education [2018GRFX023, 2018GRGDYX024]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [18KJB320004]
Recent studies have confirmed that the peptide fractions derived from marine organisms exhibit good antioxidant and anti-inflammatory activity, and oyster is an excellent nutrient resource with high-protein content. In this study, the peptide fractions from oyster soft tissue were prepared after hydrolysis by pepsin (pH 2, 37 degrees C), trypsin (pH 8, 37 degrees C), and Maxipro PSP (pH 4.2, 50 degrees C) with the optimized parameters (enzyme-to-substrate (E/S) ratio, 1:100 (w/w); hydrolysis time, 4 hr), respectively. Four fractions named as PEP-1, PEP-2, TRYP-2, and MIX-2 were obtained after separation with elution consisting of 20% or 40% ethanol. The MIX-2 exhibited the highest hydrophobicity correlated well with its hydrophobic amino acid content, and TRYP-2 exhibited much better antioxidant activity than other three elution samples. Furthermore, all of the bioactive peptide fractions were noncytotoxic and could selectively repress pro-inflammatory mediators, TNF-alpha, IL-1 beta, IL-6, and i-NOS, at transcription level in RAW264.7 macrophage cells after LPS stimulation. The result suggests that the peptide fraction TRYP-2 from oyster soft tissue hydrolysates might be a potential resource for natural anti-inflammatory components.
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